Trypsin Inhibitor Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-78088

Novus Biologicals
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Key Product Details

Species Reactivity

Plant

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Trypsin Inhibitor Primary Antibodies »

Product Specifications

Immunogen

Trypsin Inhibitor [Lima Bean] (Uniprot: P01070)

Reactivity Notes

Cross reactivity against Trypsin Inhibitor from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Trypsin Inhibitor Antibody - BSA Free

Western Blot: Trypsin Inhibitor Antibody [NBP1-78088]

Western Blot: Trypsin Inhibitor Antibody [NBP1-78088]

Western Blot: Trypsin Inhibitor Antibody [NBP1-78088] - Analysis using the Biotin conjugate of NBP1-78088. Detection of Lane 1: ~1 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 2: 0.25 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 3: ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced. Lane 4: 0.25 ug of purified Soybean Trypsin Inhibitor Non-reduced. Primary antibody: Soybean Trypsin Inhibitor antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Streptavidin, 1:5K and Atto 425 conjugated goat anti rabbit, 1:10K at 1.5 hr at RT. Block overnight at 4C. Predicted/Observed size: 24 kDa, 17 kDa for Trypsin.
Western Blot: Trypsin Inhibitor Antibody [NBP1-78088]

Western Blot: Trypsin Inhibitor Antibody [NBP1-78088]

Western Blot: Trypsin Inhibitor Antibody [NBP1-78088] - Analysis using the Biotin conjugate of NBP1-78088. Detection of Lane 1: ~1 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 2: 0.25 ug of purified Soybean Trypsin Inhibitor Reduced. Lane 3: ~1 ug of purified Soybean Trypsin Inhibitor Non-reduced. Lane 4: 0.25 ug of purified Soybean Trypsin Inhibitor Non-reduced.

Applications for Trypsin Inhibitor Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:25000

Western Blot

1:500-1:3000
Application Notes
This product has been assayed against 1.0 ug of Trypsin Inhibitor [Lima Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:15,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Trypsin Inhibitor

The soybean trypsin inhibitor was first crystallized by Kunitz in 1945 and is one of several such inhibitors found in soybeans. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly inhibited by soy inhibitor.

Alternate Names

KTI3, Kunitz trypsin inhibitor

Gene Symbol

KTI3

UniProt

P01070 (Plant)

Additional Trypsin Inhibitor Products

Product Documents for Trypsin Inhibitor Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Trypsin Inhibitor Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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