Trypsinogen Antibody - BSA Free

Novus Biologicals | Catalog # NB600-615

Novus Biologicals

Key Product Details

Species Reactivity

Bovine

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Trypsinogen Primary Antibodies »

Product Specifications

Immunogen

Trypsinogen [Bovine Pancreas] (Uniprot: Q29463)

Reactivity Notes

Cross reactivity against Trypsinogen from other tissues and species may occur but have not been specifically determined

Localization

Secreted protein; extracellular space

Specificity

Cross reactivity against Trypsinogen from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Trypsinogen Antibody - BSA Free

Western Blot: Trypsinogen Antibody [NB600-615]

Western Blot: Trypsinogen Antibody [NB600-615]

Western Blot: Trypsinogen Antibody [NB600-615] - Analysis using HRP conjugate of NB600-615. Lane 1: Trypsinogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 24 kDa, 24 kDa for Trypsinogen. Other band(s): None.

Applications for Trypsinogen Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:25000

Western Blot

1:500-1:3000
Application Notes
This product has been tested by ELIDA and dot blot and is assayed against 1.0 ug of Trypsinogen [Bovine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,300 to 1:8,400 of the reconstitution concentration is suggested for this product.

Reviewed Applications

Read 1 review rated 4 using NB600-615 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Trypsinogen

Trypsinogen has been shown to have certain intrinsic activity. It is usually considered, however, as the inactive precursor of trypsin which may be activated by removal of a terminal hexapeptide to yield single-chain b-trypsin. Subsequent limited autolysis produces other active forms having two or more peptide chains bound by disulfide bonds. The predominant forms are a-trypsin, having two peptide chains and b-, a single chain. Different activity and thermal stability are shown by a- and b-trypsin.

Alternate Names

2, Brain trypsinogen, Cationic Trypsinogen, Mesotrypsinogen, Pancreatic anionic trypsinogen, Protease, PRSS2, PRSS3, PRSS4, serine, TRP 1, TRP1, TRY1, TRY2, TRY3. TRY4, TRYP1, TRYP2, TRYP8, Trypsin 2, Trypsin 3, Trypsin III, Trypsin IV

UniProt

Q29463 (Bovine)

Additional Trypsinogen Products

Product Documents for Trypsinogen Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Trypsinogen Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Trypsinogen Antibody - BSA Free

Customer Reviews for Trypsinogen Antibody - BSA Free (1)

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  • Trypsinogen Antibody
    Name: Nisreen Chahid
    Application: Western Blot
    Sample Tested: Komagataella phaffii
    Species: Sus scrofa
    Verified Customer | Posted 10/23/2023
    Recombinant Sus scrofa Trypsinogen produced in Komagataella phaffii. Lanes 1-6: cell lysates, lanes 8-11: cell culture supernatant. Primary antibody dilution used: 1 in 250.
    Trypsinogen Antibody - BSA Free NB600-615
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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