Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Novus Biologicals | Catalog # NBP2-46646

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2990

Format

BSA Free
View all Tryptophan Hydroxylase 2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q8IWU9, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of rat midbrain shows strong positivity in serotonin neurons in the dorsal raphe nucleus.
Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of mouse brain shows moderate positivity in serotonergic fibers in the basal forebrain.
Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of mouse brain shows moderate positivity in serotonergic fibers in the cerebral cortex.
Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human dorsal raphe nucleus shows strong cytoplasmic positivity in serotonin neurons.
Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human cerebral cortex shows moderate positivity in serotonergic neural fibers.
Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human kidney shows no positivity in cells in tubules or glomeruli as expected.

Applications for Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tryptophan Hydroxylase 2

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Alternate Names

NTPH, TPH2

Entrez Gene IDs

121278 (Human)

Gene Symbol

TPH2

UniProt

Q8IWU9 (Human)

Additional Tryptophan Hydroxylase 2 Products

Product Documents for Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

There are currently no reviews for this product. Be the first to review Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free and earn rewards!

Have you used Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies