TSC2 Antibody (7X2C6)

Novus Biologicals | Catalog # NBP3-15763

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7X2C6 expressed in HEK293
View all TSC2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1700-1807 of human Tuberin (P49815). AKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

201 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TSC2 Antibody (7X2C6)

Western Blot: TSC2 Antibody (7X2C6) [NBP3-15763]

Western Blot: TSC2 Antibody (7X2C6) [NBP3-15763]

Western Blot: TSC2 Antibody (7X2C6) [NBP3-15763] - Western blot analysis of extracts of various cell lines, using TSC2 antibody (NBP3-15763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunoprecipitation: TSC2 Antibody (7X2C6) [NBP3-15763]

Immunoprecipitation: TSC2 Antibody (7X2C6) [NBP3-15763]

Immunoprecipitation: TSC2 Antibody (7X2C6) [NBP3-15763] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug TSC2 antibody (NBP3-15763). Western blot was performed from the immunoprecipitate using TSC2 antibody (NBP3-15763) at a dilition of 1:1000.

Applications for TSC2 Antibody (7X2C6)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells.

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TSC2

Tuberous sclerosis complex-2 (TCS2 also known as Tuberin) is a tumor suppressor that forms a complex with TSC1 (Hamartin ) and this complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The C-terminal region of TSC2 contains a GTPase-activating protein (GAP) domain which interacts with Rap1, Rab5 and Rheb (2). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (3). Additionally, binding of 14-3-3 beta to TSC2 at Ser1210 reduce the ability of the complex to inhibit phosphorylation of S6 kinase (4). Phosphorylation by ATK at Ser924 and Thr1518 inactivates TSC2 and disrupts its interaction with TSC1 (5). Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 individuals, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene.

Long Name

Tuberous Sclerosis 2

Alternate Names

LAM, TSC4, Tuberin

Gene Symbol

TSC2

Additional TSC2 Products

Product Documents for TSC2 Antibody (7X2C6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TSC2 Antibody (7X2C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons

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