TSFM Antibody (JE64-29)

Western Blot: TSFM Antibody (JE64-29) [NBP3-32294]

Western Blot: TSFM Antibody (JE64-29) [NBP3-32294] - Western blot analysis of TSFM on different lysates with Rabbit anti-TSFM antibody (NBP3-32294) at 1/500 dilution.

Lane 1: HepG2 cell lysate, 10 ug/Lane
Lane 2: Jurkat cell lysate, 10 ug/Lane
Lane 3: K562 cell lysate, 10 ug/Lane
Lane 4: Mouse liver tissue lysate, 20 ug/Lane
Lane 5: Mouse kidney tissue lysate, 20 ug/Lane

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 1 minute;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32294) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TSFM Antibody (JE64-29) [NBP3-32294]

Immunohistochemistry: TSFM Antibody (JE64-29) [NBP3-32294] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TSFM antibody (NBP3-32294) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32294) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: TSFM Antibody (JE64-29) [NBP3-32294]

Western blot analysis on different lysates with NBP3-32294 at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Mouse liver tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 43 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP3-32294, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 35 kDa Observed band size: 35 kDa