TSPAN7/TM4SF2 Antibody (B2D) - BSA Free
Novus Biologicals | Catalog # NBP2-75971
![Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]](https://resources.rndsystems.com/images/products/TSPAN7-TM4SF2-Antibody-B2D-BSA-Free-Flow-Cytometry-NBP2-75971-img0004.jpg "Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # B2D
Format
BSA Free
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Product Specifications
Immunogen
Human T-ALL cell line THP-6
Specificity
The antibody QBEnd-10 reacts with Class II epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TSPAN7/TM4SF2 Antibody (B2D) - BSA Free
Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]
Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971] - Analysis (surface staining) of CD231 in JURKAT cells with anti-CD231 (B2D) purified, GAM-APC![Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]](https://resources.rndsystems.com/images/products/TSPAN7-TM4SF2-Antibody-B2D-Flow-Cytometry-NBP2-75971-img0001.jpg "Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]")
Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]
Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) [NBP2-75971] - Surface staining of CD231 in JURKAT cells with anti-CD231 (B2D) purified, GAM-APC.![Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]](https://resources.rndsystems.com/images/products/TSPAN7-TM4SF2-Antibody-B2D-Flow-Cytometry-NBP2-75971-img0002.jpg "Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]")
Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]
Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) [NBP2-75971] - Surface staining of CD231 in JURKAT cells with anti-CD231 (B2D) PE.![Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]](https://resources.rndsystems.com/images/products/TSPAN7-TM4SF2-Antibody-B2D-Flow-Cytometry-NBP2-75971-img0003.jpg "Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]")
Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) - BSA Free [NBP2-75971]
Flow Cytometry: TSPAN7/TM4SF2 Antibody (B2D) [NBP2-75971] - Surface staining of CD231 in JURKAT cells with anti-CD231 (B2D) FITC.Applications for TSPAN7/TM4SF2 Antibody (B2D) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-4 ug/ml
Immunohistochemistry
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
1:100-1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Phosphate buffered saline (PBS), pH 7.4
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TSPAN7
Long Name
Tetraspanin 7
Alternate Names
A15, CCG-B7, CD231, DXS1692E, MRX58, MXS1, TALLA-1, TM4SF2, TM4SF2b
Gene Symbol
TSPAN7
Additional TSPAN7 Products
Product Documents for TSPAN7/TM4SF2 Antibody (B2D) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TSPAN7/TM4SF2 Antibody (B2D) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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