TTC33 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-37758
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 212 to 262 of human Tetratricopeptide Repeat Domain 33 using the numbering given in entry NP_036514.1 (GeneID 23548).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TTC33 Antibody - BSA Free
Western Blot: TTC33 Antibody [NBP2-37758]
Western Blot: TTC33 Antibody [NBP2-37758] - Detection of Human TTC33 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TTC33 antibody used for IP at 6 ug per reaction. TTC33 was also immunoprecipitated by rabbit anti-TTC33 antibody. For blotting immunoprecipitated TTC33, NBP2-37758 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Western Blot: TTC33 Antibody [NBP2-37758]
Western Blot: TTC33 Antibody [NBP2-37758] - Detection of Human and Mouse TTC33 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TTC33 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for TTC33 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TTC33
Alternate Names
osmosis responsive factor, Osmosis-responsive factor, OSRF, tetratricopeptide repeat domain 33, tetratricopeptide repeat protein 33, TPR repeat protein 33
Gene Symbol
TTC33
UniProt
Additional TTC33 Products
Product Documents for TTC33 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for TTC33 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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