TUSC4 Antibody

Novus Biologicals | Catalog # NB100-93442

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence QYSNVYCPTPKV corresponding to internal region according to NP_006536.3.

Epitope

QYSNVYCPTPKV

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TUSC4 Antibody

Western Blot: TUSC4 Antibody [NB100-93442]

Western Blot: TUSC4 Antibody [NB100-93442]

Western Blot: TUSC4 Antibody [NB100-93442] - (0.1ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for TUSC4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0 1 - 0.3 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in human skeletal muscle lysates (calculated MW of 43.7 kDa band according to NP_006536.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TUSC4

Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at the Tyr-9 Tyr-373 and Tyr-376 residues. May act as a tumor suppressor. Suppresses cell growth and enhanced sensitivity to various anticancer drugs

Alternate Names

G21 protein, Gene 21 protein, homologous to yeast nitrogen permease (candidate tumor suppressor), nitrogen permease regulator 2-like protein, nitrogen permease regulator-like 2 (S. cerevisiae), NPR2, NPR2L2810446G01Rik, NPRL2, Tumor suppressor candidate 4NPR2-like protein, TUSC4

Entrez Gene IDs

10641 (Human); 56032 (Mouse)

Gene Symbol

NPRL2

Additional TUSC4 Products

Product Documents for TUSC4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TUSC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for TUSC4 Antibody

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  • Q: Would you have any data about antibody NB100-93442 used in WB on a cell lysate instead of muscle extract? In case you don't have any data for this, would you be ok to include an application of WB on human cell lysate as part of the innovator's reward program?

    A: We have not tested this antibody on cell lysates. Testing on human tissues ensures that this antibody is capable of detecting the endogenous protein. Provided that your samples are prepared properly, there should be no problem with this antibody. Unfortunately, I cannot extend the Innovator's Reward Program in this case, but this would be covered by our 100% guarantee.

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