TXNDC12 Antibody (9J6L2)

Novus Biologicals | Catalog # NBP3-33549

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 9J6L2
View all TXNDC12 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TXNDC12 (NP_056997.1).

Sequence:
METRPRLGATCLLGFSFLLLVISSDGHNGLGKGFGDHIHWRTLEDGKKEAAASGLPLMVIIHKSWCGACKALKPKFAESTEISELSHNFVMVNLEDEEEP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TXNDC12 Antibody (9J6L2)

TXNDC12 Antibody (9J6L2)

Immunohistochemistry: TXNDC12 Antibody (9J6L2) [NBP3-33549] -

Immunohistochemistry: TXNDC12 Antibody (9J6L2) [NBP3-33549] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TXNDC12 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
TXNDC12 Antibody (9J6L2)

Immunohistochemistry: TXNDC12 Antibody (9J6L2) [NBP3-33549] -

Immunohistochemistry: TXNDC12 Antibody (9J6L2) [NBP3-33549] - Immunohistochemistry analysis of paraffin-embedded Mouse stomach using TXNDC12 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
TXNDC12 Antibody (9J6L2)

Immunohistochemistry: TXNDC12 Antibody (9J6L2) [NBP3-33549] -

Immunohistochemistry: TXNDC12 Antibody (9J6L2) [NBP3-33549] - Immunohistochemistry analysis of paraffin-embedded Rat brain using TXNDC12 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
TXNDC12 Antibody (9J6L2)

Immunohistochemistry: TXNDC12 Antibody (9J6L2) [NBP3-33549] -

Immunohistochemistry: TXNDC12 Antibody (9J6L2) [NBP3-33549] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using TXNDC12 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
TXNDC12 Antibody (9J6L2)

Immunohistochemistry: TXNDC12 Antibody (9J6L2) [NBP3-33549] -

Immunohistochemistry: TXNDC12 Antibody (9J6L2) [NBP3-33549] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using TXNDC12 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
TXNDC12 Antibody (9J6L2)

Western Blot: TXNDC12 Antibody (9J6L2) [NBP3-33549] -

Western Blot: TXNDC12 Antibody (9J6L2) [NBP3-33549] - Western blot analysis of various lysates using at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
TXNDC12 Antibody (9J6L2)

Western Blot: TXNDC12 Antibody (9J6L2) [NBP3-33549] -

Western Blot: TXNDC12 Antibody (9J6L2) [NBP3-33549] - Western blot analysis of various lysates using at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Applications for TXNDC12 Antibody (9J6L2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TXNDC12

TXNDC12 belongs to the thioredoxin superfamily (see TXN; MIM 187700). Members of this superfamily possess a thioredoxin fold with a consensus active-site sequence (CxxC) and have roles in redox regulation, defense against oxidative stress, refolding of disulfide-containing proteins, and regulation of transcription factors (Liu et al., 2003 (PubMed 14557066)).(supplied by OMIM)

Alternate Names

AG1, AGR1, anterior gradient homolog 1, EC 1.8.4.2, endoplasmic reticulum protein ERp19, Endoplasmic reticulum resident protein 18, Endoplasmic reticulum resident protein 19, endoplasmic reticulum thioredoxin superfamily member, 18 kDa, ER protein 18, ER protein 19, ERP16, ERp18, ERP18AG1, ERP19, hAG-1, hTLP19, PDIA16, protein disulfide isomerase family A, member 16, thioredoxin domain containing 12 (endoplasmic reticulum), thioredoxin domain-containing protein 12, Thioredoxin-like protein p19, TLP19, TLP19hTLP19, TXNDC12 thioredoxin domain containing 12 (endoplasmic reticulum)

Gene Symbol

TXNDC12

Additional TXNDC12 Products

Product Documents for TXNDC12 Antibody (9J6L2)

Certificate of Analysis

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Product Specific Notices for TXNDC12 Antibody (9J6L2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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