u-Plasminogen Activator/Urokinase Antibody (JM106-09)

Novus Biologicals | Catalog # NBP2-66766

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM106-09 expressed in HEK293
View all u-Plasminogen Activator (uPA)/Urokinase Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human u-Plasminogen Activator/Urokinase aa 50-99 / 431. (SwissProt: P00749 Human; SwissProt: P06869 Mouse; SwissProt: P29598 Rat)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for u-Plasminogen Activator/Urokinase Antibody (JM106-09)

Western Blot: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Western Blot: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Western Blot: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766] - Analysis of Urokinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-3 cell lysateLane 2: MCF-7 cell lysate
Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766] - Analysis of paraffin-embedded mouse kidney tissue using anti-Urokinase antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766] - Analysis of paraffin-embedded human colon carcinoma tissue using anti-Urokinase antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766] - Analysis of paraffin-embedded human kidney tissue using anti-Urokinase antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766] - Analysis of paraffin-embedded human liver carcinoma tissue using anti-Urokinase antibody. Counter stained with hematoxylin.
u-Plasminogen Activator/Urokinase Antibody (JM106-09) Western Blot: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Western Blot: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766]

Western blot analysis of MCF7 cell lysate with NBP2-66766 at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 120 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-66766, 1/25,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 49 kDa Observed band size: 49 kDa

Applications for u-Plasminogen Activator/Urokinase Antibody (JM106-09)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: u-Plasminogen Activator (uPA)/Urokinase

Urokinase specifically cleave the zymogen plasminogen to form the active enzyme plasmin.

Long Name

Urokinase-type Plasminogen Activator

Alternate Names

PLAU, uPA, uPlasminogen Activator, Urokinase

Gene Symbol

PLAU

Additional u-Plasminogen Activator (uPA)/Urokinase Products

Product Documents for u-Plasminogen Activator/Urokinase Antibody (JM106-09)

Certificate of Analysis

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Product Specific Notices for u-Plasminogen Activator/Urokinase Antibody (JM106-09)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Blood Coagulation Signaling Pathways
Blood Coagulation Signaling Pathway Thumbnail