UBA3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-48628

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UBA3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: YPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit UBA3 Antibody - BSA Free (NBP2-48628) is a polyclonal antibody validated for use in IHC and WB. Anti-UBA3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for UBA3 Antibody - BSA Free

Western Blot: UBA3 Antibody [NBP2-48628]

Western Blot: UBA3 Antibody [NBP2-48628]

Western Blot: UBA3 Antibody [NBP2-48628] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628]

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628]

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human testis shows strong nuclear positivity.
Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628]

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628]

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human cerebellum shows moderate nuclear positivity in purkinje cells.
Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628]

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628]

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human liver shows very weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628]

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628]

Immunohistochemistry-Paraffin: UBA3 Antibody [NBP2-48628] - Staining of human skin shows moderate nuclear positivity in epidermal cells.

Applications for UBA3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBA3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

Ubiquitin-like Modifier Activating Enzyme 3

Alternate Names

UBE1C

Gene Symbol

UBA3

Additional UBA3 Products

Product Documents for UBA3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for UBA3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E1 Ubiquitin/Ubl Activating Enzymes

Citations for UBA3 Antibody - BSA Free

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Protocols

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FAQs

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