Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all UbcH2/UBE2H Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human UbcH2/UBE2H (Accession#: P62256; Met1-Leu183)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UbcH2/UBE2H Antibody - Azide and BSA Free

Western Blot: UbcH2/UBE2H Antibody [NBP3-00048]

Western Blot: UbcH2/UBE2H Antibody [NBP3-00048]

Western Blot: UbcH2/UBE2H Antibody [NBP3-00048] - Anti-UbcH2/UBE2H rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: UbcH2/UBE2H Antibody [NBP3-00048]

Immunoprecipitation: UbcH2/UBE2H Antibody [NBP3-00048]

Immunoprecipitation: UbcH2/UBE2H Antibody [NBP3-00048] - UbcH2/UBE2H was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate 2 ul anti-UbcH2/UBE2H rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-UbcH2/UBE2H rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa

Applications for UbcH2/UBE2H Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UbcH2/UBE2H

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Alternate Names

E2-14K, E2-20K, UBC8, UBE2H

Gene Symbol

UBE2H

Additional UbcH2/UBE2H Products

Product Documents for UbcH2/UBE2H Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UbcH2/UBE2H Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail