UbcH8/Ube2L6 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-00032

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all UbcH8 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human UbcH8/Ube2L6 (Accession#: O14933-1; Met1-Ser153)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UbcH8/Ube2L6 Antibody - Azide and BSA Free

Western Blot: UbcH8/Ube2L6 Antibody [NBP3-00032]

Western Blot: UbcH8/Ube2L6 Antibody [NBP3-00032]

Western Blot: UbcH8/Ube2L6 Antibody [NBP3-00032] - Anti-GILT/IFI30 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 30 kDa
Immunoprecipitation: UbcH8/Ube2L6 Antibody [NBP3-00032]

Immunoprecipitation: UbcH8/Ube2L6 Antibody [NBP3-00032]

Immunoprecipitation: UbcH8/Ube2L6 Antibody [NBP3-00032] - UbcH8/Ube2L6 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 2 ul anti-UbcH8/Ube2L6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-UbcH8/Ube2L6 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 18 kDa

Applications for UbcH8/Ube2L6 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UbcH8

Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene.

Alternate Names

RIG-B, UBE2L6

Gene Symbol

UBE2L6

Additional UbcH8 Products

Product Documents for UbcH8/Ube2L6 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for UbcH8/Ube2L6 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UbcH8/Ube2L6 Antibody - Azide and BSA Free

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail