UBE2J1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-78065

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This affinity purified UBE2J1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the amino terminus of the human UBE2J1 protein. (Uniprot: Q9Y385)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with UBE2J1 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Localization

Endoplasmic reticulum

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This affinity purified antibody is directed against human UBE2J1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for UBE2J1 Antibody - BSA Free

Western Blot: UBE2J1 Antibody [NBP1-78065]

Western Blot: UBE2J1 Antibody [NBP1-78065]

Western Blot: UBE2J1 Antibody [NBP1-78065] - Western blot using affinity purified UBE2J1 antibody shows detection of UBE2J1 in 293 cells over-expressing Myc-UBE2J1 (Lane 2). Lane 1 contains lysate from mock-transfected 293 cells.
N/A: UBE2J1 Antibody [NBP1-78065]
UBE2J1 Antibody

UBE2J1 Antibody

Western blot using affinity purified anti-Ube2j1 antibody shows detection of Ube2j1 in 293 cells over-expressing Myc-Ube2j1 (Lane 2). Lane 1 contains lysate from mock-transfected 293 cells. Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD

Applications for UBE2J1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000-1:5000

Immunoprecipitation

1-2 ug

Western Blot

1:200-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35-40 kDa in size corresponding to Ube2j1 protein by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: UBE2J1

Ube2j1 and Ube2j2 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-can

Long Name

Ubiquitin-conjugating Enzyme E2J 1

Alternate Names

CGI-76, HSPC153, NCUBE1, UBC6e

Entrez Gene IDs

51465 (Human)

Gene Symbol

UBE2J1

UniProt

Additional UBE2J1 Products

Product Documents for UBE2J1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for UBE2J1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail