UBE2K/E2-25K Antibody (1D10) - Azide and BSA Free

Novus Biologicals | Catalog # H00003093-M13

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D10

Format

Azide and BSA Free
View all UBE2K/E2-25K Primary Antibodies »

Product Specifications

Immunogen

UBE2K (-, 1 a.a. ~ 40 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLAMSPPAADSYPPPPPPLRPPPPPPPLPPPPPPSAIQT*

Specificity

UBE2K - ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast) (1D10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for UBE2K/E2-25K Antibody (1D10) - Azide and BSA Free

Sandwich ELISA: UBE2K/E2-25K Antibody (1D10) [H00003093-M13] - Detection limit for recombinant GST tagged UBE2K is 0.1 ng/ml as a capture antibody.

Applications for UBE2K/E2-25K Antibody (1D10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE2K/E2-25K

The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Ubiquitin-conjugating Enzyme E2

Alternate Names

E2-25K, E225K, Hip2, HYPG, LIG, UbcH1, UBE2K, UBE2K-25K

Entrez Gene IDs

3093 (Human)

Gene Symbol

UBE2K

UniProt

BC006419 (Human)

Additional UBE2K/E2-25K Products

Product Documents for UBE2K/E2-25K Antibody (1D10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UBE2K/E2-25K Antibody (1D10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail