![Immunocytochemistry/ Immunofluorescence: UBE2O Antibody [NBP2-20787]](https://resources.rndsystems.com/images/products/UBE2O-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20787-img0004.jpg "Immunocytochemistry/ Immunofluorescence: UBE2O Antibody [NBP2-20787]")
Loading...
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Loading...
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human UBE2O. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for UBE2O Antibody
Immunocytochemistry/ Immunofluorescence: UBE2O Antibody [NBP2-20787]
Immunocytochemistry/Immunofluorescence: UBE2O Antibody [NBP2-20787] - Immunofluorescence analysis of methanol-fixed A431, using antibody at 1:500 dilution.![Immunohistochemistry-Paraffin: UBE2O Antibody [NBP2-20787]](https://resources.rndsystems.com/images/products/UBE2O-Antibody-Immunohistochemistry-Paraffin-NBP2-20787-img0003.jpg "Immunohistochemistry-Paraffin: UBE2O Antibody [NBP2-20787]")
Immunohistochemistry-Paraffin: UBE2O Antibody [NBP2-20787]
Immunohistochemistry-Paraffin: UBE2O Antibody [NBP2-20787] - UBE2O antibody [C1C2], Internal detects UBE2O protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. UBE2O antibody [C1C2], Internal dilution: 1:500.![Immunohistochemistry-Paraffin: UBE2O Antibody [NBP2-20787]](https://resources.rndsystems.com/images/products/UBE2O-Antibody-Immunohistochemistry-Paraffin-NBP2-20787-img0002.jpg "Immunohistochemistry-Paraffin: UBE2O Antibody [NBP2-20787]")
Immunohistochemistry-Paraffin: UBE2O Antibody [NBP2-20787]
Immunohistochemistry-Paraffin: UBE2O Antibody [NBP2-20787] - Immunohistochemical analysis of paraffin-embedded breast ca, using antibody at 1:500 dilution.
Western Blot: UBE2O Antibody [NBP2-20787] -
Western Blot: UBE2O Antibody [NBP2-20787] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with UBE2O antibody [C1C2], Internal (NBP2-20787) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for UBE2O Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
10 - 1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: UBE2O
Alternate Names
E2-230K, EC 6.3.2.19, FLJ12878, KIAA1734, Ubiquitin carrier protein O, ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-conjugating enzyme E2-230K, ubiquitin-conjugating enzyme E2O, Ubiquitin-protein ligase O
Gene Symbol
UBE2O
UniProt
Additional UBE2O Products
Product Documents for UBE2O Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UBE2O Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for UBE2O Antibody
There are currently no reviews for this product. Be the first to review UBE2O Antibody and earn rewards!
Have you used UBE2O Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...