UBE2R2 Antibody (5E7) - Azide and BSA Free

Novus Biologicals | Catalog # H00054926-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5E7

Format

Azide and BSA Free
View all UBE2R2 Primary Antibodies »

Product Specifications

Immunogen

UBE2R2 (AAH47584, 1 a.a. ~ 238 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKEYAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSNDNSSDLLYDDLYDDDIDDEDEEEEDADCYDDDDSGNEES

Reactivity Notes

Human. Other species not tested.

Specificity

UBE2R2 - ubiquitin-conjugating enzyme E2R 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for UBE2R2 Antibody (5E7) - Azide and BSA Free

Western Blot: UBE2R2 Antibody (5E7) [H00054926-M01]

Western Blot: UBE2R2 Antibody (5E7) [H00054926-M01]

Western Blot: UBE2R2 Antibody (5E7) [H00054926-M01] - UBE2R2 monoclonal antibody (M01), clone 5E7 Analysis of UBE2R2 expression in Jurkat.
Western Blot: UBE2R2 Antibody (5E7) [H00054926-M01]

Western Blot: UBE2R2 Antibody (5E7) [H00054926-M01]

Western Blot: UBE2R2 Antibody (5E7) [H00054926-M01] - Analysis of UBE2R2 expression in transfected 293T cell line by UBE2R2 monoclonal antibody (M01), clone 5E7.Lane 1: UBE2R2 transfected lysate (Predicted MW: 27.2 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: UBE2R2 Antibody (5E7) [H00054926-M01]

Immunoprecipitation: UBE2R2 Antibody (5E7) [H00054926-M01]

Immunoprecipitation: UBE2R2 Antibody (5E7) [H00054926-M01] - Analysis of UBE2R2 transfected lysate using anti-UBE2R2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UBE2R2 monoclonal antibody.
Sandwich ELISA: UBE2R2 Antibody (5E7) [H00054926-M01] - Detection limit for recombinant GST tagged UBE2R2 is approximately 0.1ng/ml as a capture antibody.

Applications for UBE2R2 Antibody (5E7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE2R2

Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.

Long Name

Ubiquitin-conjugating Enzyme E2R 2

Alternate Names

CDC34B, UBC3B

Entrez Gene IDs

54926 (Human)

Gene Symbol

UBE2R2

UniProt

AAH47584 (Human)

Additional UBE2R2 Products

Product Documents for UBE2R2 Antibody (5E7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UBE2R2 Antibody (5E7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail