UBE2S Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20788

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (99%), Rat (99%), Rhesus Macaque (100%), Xenopus (99%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE2S. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (99%), Zebrafish (81%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2S Antibody - BSA Free

Western Blot: UBE2S Antibody [NBP2-20788]

Western Blot: UBE2S Antibody [NBP2-20788]

Western Blot: UBE2S Antibody [NBP2-20788] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.
UBE2S Antibody - BSA Free

Western Blot: UBE2S Antibody [NBP2-20788] -

Wild-type (WT) and UBE2S knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with UBE2S antibody [N1C2] (NBP2-20788) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for UBE2S Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-20788 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE2S

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq]

Long Name

Ubiquitin-conjugating Enzyme E2S

Alternate Names

E2-24K, E2-EPF, E2-EPF52, EPF5

Gene Symbol

UBE2S

UniProt

Additional UBE2S Products

Product Documents for UBE2S Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for UBE2S Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for UBE2S Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
5 Stars
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4 Stars
100%
3 Stars
0%
2 Stars
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1 Stars
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Customer Images


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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 02/17/2016
    western blotting image for Ube2S in HeLa cell lysate
    UBE2S Antibody - BSA Free NBP2-20788

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FAQs for UBE2S Antibody - BSA Free

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  • Q: I am interested in your Ube2s antibody, NBP2-20788, and would like to clarify what the immunogen is. You specify that the immuogen is a Recombinant fragment corresponding to a region within amino acids 1 and 185 of UBE2S. Does this mean this whole fragment comprising amino acids 1-185 was used as an immunogen or are you referring to a smaller region within residues 1-185? If the latter is true, what exactly is the region within 1-185 that was used as immunogen?

    A: The immunogen given as 1-185 is just the amino acid range for the actual immunogen. The exact sequence is proprietary. If you would like us to help you align a specific sequence, we would be happy to do that for you.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail