UBE2T Antibody

Novus Biologicals | Catalog # NB100-1150

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-QLVGIEKKFHPDV corresponding to C-Terminus according to NP_054895.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for UBE2T Antibody

Western Blot: UBE2T Antibody [NB100-1150]

Western Blot: UBE2T Antibody [NB100-1150]

Western Blot: UBE2T Antibody [NB100-1150] - Analysis of UBE2T in K562 lysate (35ug protein in RIPA buffer) using NB100-1150 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for UBE2T Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 22 kDa band observed in lysates of K562, HEK293 and A549 (calculated MW of 22.5 kDa band according to NP_054895.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2T

The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM]

Long Name

Ubiquitin-conjugating Enzyme E2T

Alternate Names

HSPC150, PIG50

Entrez Gene IDs

29089 (Human)

Gene Symbol

UBE2T

Additional UBE2T Products

Product Documents for UBE2T Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for UBE2T Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail