UBE4B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84943

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UBE4B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPI

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22864569)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE4B Antibody - BSA Free

Western Blot: UBE4B Antibody [NBP1-84943]

Western Blot: UBE4B Antibody [NBP1-84943]

Western Blot: UBE4B Antibody [NBP1-84943] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: UBE4B Antibody [NBP1-84943]

Immunohistochemistry-Paraffin: UBE4B Antibody [NBP1-84943]

Immunohistochemistry-Paraffin: UBE4B Antibody [NBP1-84943] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Western Blot: UBE4B Antibody [NBP1-84943]

Western Blot: UBE4B Antibody [NBP1-84943]

Western Blot: UBE4B Antibody [NBP1-84943] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Applications for UBE4B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE4B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes.

Long Name

Ubiquitination Factor E4B

Alternate Names

UBOX3, UFD2, UFD2a

Gene Symbol

UBE4B

Additional UBE4B Products

Product Documents for UBE4B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UBE4B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

Citations for UBE4B Antibody - BSA Free

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Protocols

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FAQs

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