Ubiquitin-activating Enzyme/UBE1 Antibody

Novus Biologicals | Catalog # NB100-58804

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human ubiquitin-activating enzyme E1(UBE1) using the numbering given in entry NP_003325.2 (GeneID 7317).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ubiquitin-activating Enzyme/UBE1 Antibody

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB100-58804]

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB100-58804]

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB100-58804] - Detection of Human and Mouse UBE1 on HeLa whole cell lysate using NB100-58804. UBE1 was also IPed efficiently by rabbit anti-UBE1 antibodies NB100-58805 and NB100-58806.

Applications for Ubiquitin-activating Enzyme/UBE1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ubiquitin-activating Enzyme/UBE1

E1 Ubiquitin Activating Enzyme is encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.

Alternate Names

A1S9T, AMCX1, GXP1, POC20, SMAX2, UBA1, UBE1, Ubiquitinactivating Enzyme

Entrez Gene IDs

7317 (Human); 22201 (Mouse)

Gene Symbol

UBA1

Additional Ubiquitin-activating Enzyme/UBE1 Products

Product Documents for Ubiquitin-activating Enzyme/UBE1 Antibody

Certificate of Analysis

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Product Specific Notices for Ubiquitin-activating Enzyme/UBE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail