UBR5/EDD Antibody - BSA Free

Novus Biologicals | Catalog # NB100-1591

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UBR5 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human E3 Ubiquitin Protein Ligase, HECTdomain containing, 1 using the numbering given in entry NP_056986.2 (GeneID 51366)

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

309 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBR5/EDD Antibody - BSA Free

Western Blot: UBR5/EDD Antibody [NB100-1591]

Western Blot: UBR5/EDD Antibody [NB100-1591]

Western Blot: UBR5/EDD Antibody [NB100-1591] - Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591]

Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591]

Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591]

Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591]

Immunohistochemistry-Paraffin: UBR5/EDD Antibody [NB100-1591] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB
Immunoprecipitation: UBR5/EDD Antibody [NB100-1591]

Immunoprecipitation: UBR5/EDD Antibody [NB100-1591]

Immunoprecipitation: UBR5/EDD Antibody [NB100-1591] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit UBR5/EDD Antibody (NB100-1591, lot 6) used for IP at 6 ug per reaction. UBR5/EDD was also immunoprecipitated by a previous lot of this antibody (lot 5). For blotting immunoprecipitated UBR5/EDD, NB100-1591 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 minutes.

Applications for UBR5/EDD Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg of lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UBR5

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. EDD1 has also been shown to associate with Chk2 and is necessary for the efficient phosphorylation and activation of Chk2 in response to DNA damage.

Long Name

Ubiquitin Protein Ligase E3 Component N-recognin 5

Alternate Names

DD5, EDD, EDD1, HYD

Entrez Gene IDs

51366 (Human); 70790 (Mouse)

Gene Symbol

UBR5

UniProt

NP_056986.2 (Human)

Additional UBR5 Products

Product Documents for UBR5/EDD Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for UBR5/EDD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

Citations for UBR5/EDD Antibody - BSA Free

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Protocols

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