UGT1A9 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00054600-B01P

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all UGT1A9 Primary Antibodies »

Product Specifications

Immunogen

UGT1A9 (NP_066307.1, 1 a.a. - 530 a.a.) full-length human protein. MACTGWTSPLPLCVCLLLTCGFAEAGKLLVVPMDGSHWFTMRSVVEKLILRGHEVVVVMPEVSWQLGRSLNCTVKTYSTSYTLEDLDREFKAFAHAQWKAQVRSIYSLLMGSYNDIFDLFFSNCRSLFKDKKLVEYLKESSFDAVFLDPFDNCGLIVAKYFSLPSVVFARGILCHYLEEGAQCPAPLSYVPRILLGFSDAMTFKERVRNHIMHLEEHLLCHRFFKNALEIASEILQTPVTEYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGINCHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23055538)

Specificity

UGT1A9 - UDP glucuronosyltransferase 1 family, polypeptide A9,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for UGT1A9 Antibody - Azide and BSA Free

Western Blot: UGT1A9 Antibody [H00054600-B01P]

Western Blot: UGT1A9 Antibody [H00054600-B01P]

Western Blot: UGT1A9 Antibody [H00054600-B01P] - Analysis of UGT1A9 expression in human liver.
Immunohistochemistry-Paraffin: UGT1A9 Antibody [H00054600-B01P]

Immunohistochemistry-Paraffin: UGT1A9 Antibody [H00054600-B01P]

Immunohistochemistry-Paraffin: UGT1A9 Antibody [H00054600-B01P] - Analysis of purified antibody to UGT1A9 on formalin-fixed paraffin-embedded human small Intestine. (antibody concentration 3 ug/ml)
Western Blot: UGT1A9 Antibody [H00054600-B01P]

Western Blot: UGT1A9 Antibody [H00054600-B01P]

Western Blot: UGT1A9 Antibody [H00054600-B01P] - Analysis of UGT1A9 expression in transfected 293T cell line by UGT1A9 polyclonal antibody. Lane 1: UGT1A9 transfected lysate(58.3 KDa). Lane 2: Non-transfected lysate.

Applications for UGT1A9 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. Has also been used for immunohistochemistry (paraffin).

Reviewed Applications

Read 1 review rated 4 using H00054600-B01P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UGT1A9

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenols. [provided by RefSeq]

Long Name

UDP Glucuronosyltransferase 1A9

Alternate Names

A12, LUGP4, UDPGT, UDPGT1-9, UGT1-09, UGT1-9, UGT1.9, UGT1I

Entrez Gene IDs

54600 (Human)

Gene Symbol

UGT1A9

UniProt

NP_066307.1 (Human)

Additional UGT1A9 Products

Product Documents for UGT1A9 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UGT1A9 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

Citations for UGT1A9 Antibody - Azide and BSA Free

Customer Reviews for UGT1A9 Antibody - Azide and BSA Free (1)

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  • UGT1A9 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Hepa 1-6 mouse hepatoma cell line
    Species: Mouse
    Verified Customer | Posted 03/16/2021
    UGT1A9 Antibody H00054600-B01P 1:1000 in 1% skim milk/TBST, overnight (4°C) Sample: Hepa 1-6 cell lysate: mouse hepatocellular carcinoma 10 micro gram/each lane
    Blocking step: Milk as blocking agent for 1 hour; Concentration 5 %; Temperature 25°C 1st Ab Incubation time: 16 hours, Temperature 4°C; Diluent: 1% skim milk in TBST
    UGT1A9 Antibody - Azide and BSA Free H00054600-B01P

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Protocols

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