ULK1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-94753

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ULK1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human ULK1 (NP_003556.1). SSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit ULK1 Antibody - Azide and BSA Free (NBP2-94753) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ULK1 Antibody - Azide and BSA Free

ULK1 Antibody - Azide and BSA Free

Western Blot: ULK1 Antibody - Azide and BSA Free [NBP2-94753] -

Western Blot: ULK1 Antibody - Azide and BSA Free [NBP2-94753] - Western blot analysis of various lysates, using ULK1 Rabbit pAb (A8529) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western Blot: ULK1 AntibodyAzide and BSA Free [NBP2-94753]

Western Blot: ULK1 AntibodyAzide and BSA Free [NBP2-94753]

Western Blot: ULK1 Antibody [NBP2-94753] - Western blot analysis of extracts of Rat brain, using ULK1 antibody (NBP2-94753) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
ULK1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ULK1 Antibody - Azide and BSA Free [NBP2-94753] -

Immunocytochemistry/ Immunofluorescence: ULK1 Antibody - Azide and BSA Free [NBP2-94753] - Immunofluorescence analysis of NIH/3T3 cells using ULK1 Rabbit pAb (A8529) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ULK1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ULK1 Antibody - Azide and BSA Free [NBP2-94753] -

Immunocytochemistry/ Immunofluorescence: ULK1 Antibody - Azide and BSA Free [NBP2-94753] - Immunofluorescence analysis of HeLa cells using ULK1 Rabbit pAb (A8529) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for ULK1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ULK1

ULK1 belongs to the serine/threonine protein kinase family. It is involved in axon growth and plays an essential role in neurite branching during sensory axon outgrowth. Knockdown of ULK1 results in impaired endocytosis of nerve growth factor (NGF), excessive axon arborization, and severely stunted axon elongation indicating that ULK1 mediates a non clathrin coated endocytosis in sensory growth cones. Knockdown of ULK1 also inhibits the autophagic response. It appears to act as a convergence point for multiple signals that regulate autophagy, and in turn interacts with a large number of autophagy related (Atg) proteins.

Long Name

Serine/threonine-protein kinase ULK1

Alternate Names

ATG1, hATG1, KIAA0722

Gene Symbol

ULK1

Additional ULK1 Products

Product Documents for ULK1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ULK1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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