UNG Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-49985

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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation (Negative), Knockdown Validated

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UNG Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 263 and 313 of human Uracil-DNA Glycosylase using the numbering given in entry NP_550433.1 (GeneID 7374).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UNG Antibody - BSA Free

Knockdown Validated: UNG Antibody [NBP1-49985]

Western Blot: UNG Antibody [NBP1-49985]

Western Blot: UNG Antibody [NBP1-49985] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and UNG knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human UNG Polyclonal Antibody (Catalog # NBP1-49985) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UNG at approximately 38 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: UNG Antibody [NBP1-49985]

Western Blot: UNG Antibody [NBP1-49985]

Western Blot: UNG Antibody [NBP1-49985] - Detection of Human UNG by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UNG antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. UNG was successfully immunoprecipitated by rabbit anti-UNG antibody which recognizes an upstream epitope. For blotting immunoprecipitated UNG was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
UNG Antibody - BSA Free

Western Blot: UNG Antibody - BSA Free [NBP1-49985] -

VLP-delivered HA-Vpr induces a proteasome-dependent decrease in human B lymphocyte uracil removal capacity. a Daudi B-cells were transduced with VLP HA-Vpr wt or mutant (R90K, Q65R or W54R) or delta Vpr at M.O.I of 10. 72 h later cells were lysed and examined by immunoblot for UNG2 content (with anti-UNG2 antibody or anti-alpha tubulin as control). Lanes were cropped from different parts of a unique gel (see Additional file 2: Supplementary dataset 2). Corresponding UNG activity was measured in the presence or absence of the UNG inhibitor UGI. b Daudi cells were treated for 1 h with 50 uM MG132 and then transduced with the indicated VLPs at MOI of 10. 24 h later, UNG2 content was analyzed with anti-UNG2 by western blotting (lanes were cropped from different parts of a unique gel (see Additional file 2: Supplementary dataset 2)) and UNG activity as in (a). Values are the means of triplicate measurements +/- SD. Statistical significance was determined using the ANOVA test. Ut: untransduced Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32778120), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for UNG Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP1-49985 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UNG

The human UNG-gene encodes nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase using differentially regulated promoters, PA and PB, and alternative splicing to produce two proteins with unique N-terminal sorting sequences. Uracil in DNA may result from deamination of cytosine which can give rise to transition mutations. Uracil-DNA glycosylase is the DNA repair enzyme responsible for the removal of uracil from DNA. This is probably a biologically important function in the prevention of mutagenesis resulting from cytosine deamination. UNG2 removes misincorporated dUMP residues.

Long Name

Uracil N-Glycosylase

Alternate Names

DGU, UDG

Gene Symbol

UNG

Additional UNG Products

Product Documents for UNG Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for UNG Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

Citations for UNG Antibody - BSA Free

Customer Reviews for UNG Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Whole cell lysate, Sample Amount: 25 ug
    Species: Human
    Verified Customer | Posted 12/01/2011
    UNG Antibody - BSA Free NBP1-49985

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