Rat Urocortin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-53878

Novus Biologicals

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

6.5 pg/mL (example only; lot dependent)

Assay Range

15.625 - 1000 pg/mL (example only; lot dependent)
View all Urocortin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Kit Contents for Rat Urocortin ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Urocortin

Urocortins 1, 2 and 3 are the mammalian homologs of fish urotensin I. Mammalian urocortin is a member of the corticotropin-releasing hormone(CRH) family that is expressed in a region of the rat midbrain. The distinct expression pattern in discrete brain regions suggests that it influences such behavior as feeding, anxiety and auditory processing. Synthetic human urocortin binds with high affinity to CRH receptors and acts in vitro to release ACTH from dispersed rat anterior pituitary cells. It is suggested that urocortin may be an endogenous CRF-like factor in the brain responsible for the effects of stress on appetite. The gene which encodes urocortin maps to human chromosome 2. Specifically, urocortin III is a specific ligand for CRF type 2 receptor which mediates stress-coping responses. Human urocortin I and III are expressed in the human heart. Urocortin III is also expressed in the pituitary gland, adrenal gland, GI tract, ovary, spleen, brain and kidney.

Alternate Names

UCN, UI, UROC

Gene Symbol

UCN

Additional Urocortin Products

Product Documents for Rat Urocortin ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Rat Urocortin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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