USP10 Antibody (9L6F3)

Novus Biologicals | Catalog # NBP3-16416

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9L6F3 expressed in HEK293
View all USP10 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human USP10 (Q14694). MALHSPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAVDKLPDGQEYQRIEFGVDEVIEPSDTLPRTPSYSISSTLNPQAPEFILGCTASKIT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP10 Antibody (9L6F3)

Western Blot: USP10 Antibody (9L6F3) [NBP3-16416]

Western Blot: USP10 Antibody (9L6F3) [NBP3-16416]

Western Blot: USP10 Antibody (9L6F3) [NBP3-16416] - Western blot analysis of extracts of various cell lines, using USP10 Rabbit mAb (NBP3-16416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: USP10 Antibody (9L6F3) [NBP3-16416]

Immunocytochemistry/ Immunofluorescence: USP10 Antibody (9L6F3) [NBP3-16416]

Immunocytochemistry/Immunofluorescence: USP10 Antibody (9L6F3) [NBP3-16416] - Immunofluorescence analysis of NIH-3T3 cells using USP10 Rabbit mAb (NBP3-16416) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation: USP10 Antibody (9L6F3) [NBP3-16416]

Immunoprecipitation: USP10 Antibody (9L6F3) [NBP3-16416]

Immunoprecipitation: USP10 Antibody (9L6F3) [NBP3-16416] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug USP10 antibody (NBP3-16416). Western blot was performed from the immunoprecipitate using USP10 antibody (NBP3-16416) at a dilition of 1:1000.
USP10 Antibody (9L6F3)

Immunocytochemistry/ Immunofluorescence: USP10 Antibody (9L6F3) [NBP3-16416] -

Immunocytochemistry/ Immunofluorescence: USP10 Antibody (9L6F3) [NBP3-16416] - Confocal imaging of NIH/3T3 cells using USP10 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
USP10 Antibody (9L6F3)

Immunocytochemistry/ Immunofluorescence: USP10 Antibody (9L6F3) [NBP3-16416] -

Immunocytochemistry/ Immunofluorescence: USP10 Antibody (9L6F3) [NBP3-16416] - Confocal imaging of Hep G2 cells using USP10 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Applications for USP10 Antibody (9L6F3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP10

Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene.

Long Name

Ubiquitin Specific Protease 10

Alternate Names

Ubiquitin Thiolesterase 10, UBPO, Uchrp

Gene Symbol

USP10

Additional USP10 Products

Product Documents for USP10 Antibody (9L6F3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for USP10 Antibody (9L6F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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Protocols

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FAQs

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