USP11 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80639

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry-Paraffin, Chromatin Immunoprecipitation-exo-Seq

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all USP11 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VEPQEDTRLWAKNSEGSLDRLYDTHITVLDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPGICGLTNLGNT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP11 Antibody - BSA Free

USP11 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: USP11 Antibody - BSA Free [NBP1-80639]

Chromatin Immunoprecipitation-exo-Seq: USP11 Antibody - BSA Free [NBP1-80639]

ChIP-Exo-Seq composite graph for Anti-USP11 (NBP1-80639) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
USP11 Antibody - BSA Free Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
USP11 Antibody - BSA Free Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferous ducts.
USP11 Antibody - BSA Free Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.
USP11 Antibody - BSA Free Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Staining of mouse brain shows moderate to strong positivity in neurons in the cerebral cortex.
USP11 Antibody - BSA Free Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Staining of mouse caudate putamen shows positivity in blood vessels.
USP11 Antibody - BSA Free Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.
USP11 Antibody - BSA Free Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
USP11 Antibody - BSA Free Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Immunohistochemistry-Paraffin: USP11 Antibody [NBP1-80639]

Staining of human cerebellum, cerebral cortex, skeletal muscle and testis HPA037536 (A) shows similar protein distribution across tissues to independent antibody HPA003103 (B).

Applications for USP11 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP11

Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This gene encodes a deubiquitinating enzyme which lies in a gene cluster on chromosome Xp11.23

Long Name

Ubiquitin Specific Protease 11

Alternate Names

Ubiquitin Thioesterase 11, UHX1

Entrez Gene IDs

8237 (Human)

Gene Symbol

USP11

UniProt

P51784

Additional USP11 Products

Product Documents for USP11 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for USP11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for USP11 Antibody - BSA Free

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Protocols

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