![Western Blot: USP13 Antibody [NBP2-24575]](https://resources.rndsystems.com/images/products/USP13-Antibody-Western-Blot-NBP2-24575-img0001.jpg "Western Blot: USP13 Antibody [NBP2-24575]")
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Key Product Details
Species Reactivity
Human, Mouse, Bovine, Canine, Equine, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
A portion of amino acids 500-550 of human USP13 was used as the immunogen for the antibody.
Reactivity Notes
This antibody is reactive to Chimpanzee, Rhesus Monkey.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for USP13 Antibody
Western Blot: USP13 Antibody [NBP2-24575]
Western Blot: USP13 Antibody [NBP2-24575] - analysis of human spleen lysate in the 1) absence and 2) presence of immunizing peptide using USP13 antibody at 0.25 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.![Immunohistochemistry-Paraffin: USP13 Antibody [NBP2-24575]](https://resources.rndsystems.com/images/products/USP13-Antibody-Immunohistochemistry-Paraffin-NBP2-24575-img0002.jpg "Immunohistochemistry-Paraffin: USP13 Antibody [NBP2-24575]")
Immunohistochemistry-Paraffin: USP13 Antibody [NBP2-24575]
Immunohistochemistry-Paraffin: USP13 Antibody [NBP2-24575] - Formalin-fixed, paraffin-embedded human testis stained with USP13 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Applications for USP13 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
5-10 ug/ml
Western Blot
0.25-1.0 ug/ml
Application Notes
This antibody has been tested in ELISA only.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: USP13
Long Name
Ubiquitin Specific Protease 13
Alternate Names
Isopeptidase T-3, IsoT-3, Ubiquitin Thioesterase 13
Gene Symbol
USP13
UniProt
Additional USP13 Products
Product Documents for USP13 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for USP13 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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