USP16 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86881

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all USP16 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CLVLSLDNWSVWCYVCDNEVQYCSSNQLGQVVDYVRKQASITTPKPAEKDNGNIELENKKLEKESKNEQEREKKENMAKENPPMNSPCQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKEVKM

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP16 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: USP16 Antibody [NBP1-86881]

Immunocytochemistry/ Immunofluorescence: USP16 Antibody [NBP1-86881]

Immunocytochemistry/Immunofluorescence: USP16 Antibody [NBP1-86881] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881]

Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881]

Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881]

Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881]

Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human gastrointestinal shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881]

Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881]

Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human kidney shows strong nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881]

Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881]

Immunohistochemistry-Paraffin: USP16 Antibody [NBP1-86881] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.

Applications for USP16 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP16

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 16 (USP16) is a member of this family. USP16 activity is cell cycle regulated via phosphorylation and participates in cell cycle control by influencing mitotic chromosome condensation and histone 2A (H2A) deubiquitination.

Long Name

Ubiquitin Specific Protease 16

Alternate Names

UBP-M

Gene Symbol

USP16

Additional USP16 Products

Product Documents for USP16 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for USP16 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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Protocols

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FAQs

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