USP21 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-03947

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all USP21 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human USP21 (NP_036607.3). MPQASEHRLGRTREPPVN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP21 Antibody - Azide and BSA Free

USP21 Antibody - Azide and BSA Free

Western Blot: USP21 Antibody - Azide and BSA Free [USP21] -

Western Blot: USP21 Antibody - Azide and BSA Free [USP21] - Western blot analysis of lysates from Mouse brain, using USP21 Rabbit pAb at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
USP21 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] -

Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] - Immunofluorescence analysis of PC-12 cells using USP21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
USP21 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] -

Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] - Immunofluorescence analysis of HeLa cells using USP21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
USP21 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] -

Immunocytochemistry/ Immunofluorescence: USP21 Antibody - Azide and BSA Free [USP21] - Immunofluorescence analysis of RAW264.7 cells using USP21 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for USP21 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP21

USP21 encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants have been described.

Long Name

Ubiquitin Specific Protease 21

Alternate Names

ESTM28, USP23, W53272

Gene Symbol

USP21

Additional USP21 Products

Product Documents for USP21 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for USP21 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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Protocols

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