USP47 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85942

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (97%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all USP47 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPVRKLFEDVANKVGYINGTFDLVWGNGINTADMAPLDHTSDKSLLDANFEPGK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP47 Antibody - BSA Free

USP47 Antibody - BSA Free Immunohistochemistry-Paraffin: USP47 Antibody - BSA Free [NBP1-85942]

Immunohistochemistry-Paraffin: USP47 Antibody - BSA Free [NBP1-85942]

Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
USP47 Antibody - BSA Free Western Blot: USP47 Antibody - BSA Free [NBP1-85942]

Western Blot: USP47 Antibody - BSA Free [NBP1-85942]

Analysis in human cell line K562.
USP47 Antibody - BSA Free

Western Blot: USP47 Antibody - BSA Free [NBP1-85942] -

The impact of calcification with knockdown of USP47 proteins in RASMCs. (a) The level of calcification according to real-time PCR in the control group (CTRL), the negative control group (NC), and the USP47 siRNA-transfected group (USP47 siRNA) with or without calcification (CAL). One-way analysis of variance (ANOVA) was used, *p < 0.05, **p < 0.01. (b) The expression levels of USP47, RUNX2, RANKL and SM22 alpha in USP47 siRNA RASMCs according to immunoblotting. (c) Von kossa staining of USP47 siRNA RASMCs cultured in a high phosphorus environment for 72 h. The scale bar represents 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35509185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
USP47 Antibody - BSA Free

Western Blot: USP47 Antibody - BSA Free [NBP1-85942] -

Bioinformatics analysis. (a) The protein-protein interactions network enrichment analysis. (b) The expression levels of BTRC and AKT1 in USP47 siRNA RASMCs according to immunoblotting in high phosphorus environment for 72 h. One-way analysis of variance (ANOVA) was used, *p < 0.05, **p < 0.01. (c) The levels of FGF23, Klotho (KL), and MGP in USP47 siRNA-transfected cells according to real-time PCR in high phosphorus environment for 72 h. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35509185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
USP47 Antibody - BSA Free

Immunohistochemistry: USP47 Antibody - BSA Free [NBP1-85942] -

The impact of calcification with knockdown of USP47 proteins in RASMCs. (a) The level of calcification according to real-time PCR in the control group (CTRL), the negative control group (NC), and the USP47 siRNA-transfected group (USP47 siRNA) with or without calcification (CAL). One-way analysis of variance (ANOVA) was used, *p < 0.05, **p < 0.01. (b) The expression levels of USP47, RUNX2, RANKL and SM22 alpha in USP47 siRNA RASMCs according to immunoblotting. (c) Von kossa staining of USP47 siRNA RASMCs cultured in a high phosphorus environment for 72 h. The scale bar represents 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35509185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for USP47 Antibody - BSA Free

Application
Recommended Usage

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP47

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase (USP47) is a UBP.

Long Name

Ubiquitin Specific Protease 47

Alternate Names

TRFP

Gene Symbol

USP47

Additional USP47 Products

Product Documents for USP47 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for USP47 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for USP47 Antibody - BSA Free

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