USP47 Antibody - BSA Free

Immunohistochemistry-Paraffin: USP47 Antibody - BSA Free [NBP1-85942]

Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Western Blot: USP47 Antibody - BSA Free [NBP1-85942]

Analysis in human cell line K562.

Western Blot: USP47 Antibody - BSA Free [NBP1-85942] -

The impact of calcification with knockdown of USP47 proteins in RASMCs. (a) The level of calcification according to real-time PCR in the control group (CTRL), the negative control group (NC), and the USP47 siRNA-transfected group (USP47 siRNA) with or without calcification (CAL). One-way analysis of variance (ANOVA) was used, *p < 0.05, **p < 0.01. (b) The expression levels of USP47, RUNX2, RANKL and SM22 alpha in USP47 siRNA RASMCs according to immunoblotting. (c) Von kossa staining of USP47 siRNA RASMCs cultured in a high phosphorus environment for 72 h. The scale bar represents 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35509185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: USP47 Antibody - BSA Free [NBP1-85942] -

Increased expression of USP47 in CKD vascular calcification in vivo and vitro. (a) The expression levels of USP47 as assayed by immunoblotting in a high phosphorus environment for 72 h in RASMCs. The Student’s unpaired t-test analysis was used, *p < 0.05. n = 3 for each group. (b) Alizarin red staining of CKD rat abdominal aortas and CKD patient radial arteries. (c) Representative immunohistochemical staining of USP47 in aortas of CKD rats and in the radial arteries of CKD patients. Arrows mark USP47 positive areas. The scale bar corresponds to sizes from 20 to 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35509185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: USP47 Antibody - BSA Free [NBP1-85942] -

Bioinformatics analysis. (a) The protein-protein interactions network enrichment analysis. (b) The expression levels of BTRC and AKT1 in USP47 siRNA RASMCs according to immunoblotting in high phosphorus environment for 72 h. One-way analysis of variance (ANOVA) was used, *p < 0.05, **p < 0.01. (c) The levels of FGF23, Klotho (KL), and MGP in USP47 siRNA-transfected cells according to real-time PCR in high phosphorus environment for 72 h. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35509185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: USP47 Antibody - BSA Free [NBP1-85942] -

The impact of calcification with knockdown of USP47 proteins in RASMCs. (a) The level of calcification according to real-time PCR in the control group (CTRL), the negative control group (NC), and the USP47 siRNA-transfected group (USP47 siRNA) with or without calcification (CAL). One-way analysis of variance (ANOVA) was used, *p < 0.05, **p < 0.01. (b) The expression levels of USP47, RUNX2, RANKL and SM22 alpha in USP47 siRNA RASMCs according to immunoblotting. (c) Von kossa staining of USP47 siRNA RASMCs cultured in a high phosphorus environment for 72 h. The scale bar represents 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35509185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: USP47 Antibody - BSA Free [NBP1-85942] -

Increased expression of USP47 in CKD vascular calcification in vivo and vitro. (a) The expression levels of USP47 as assayed by immunoblotting in a high phosphorus environment for 72 h in RASMCs. The Student’s unpaired t-test analysis was used, *p < 0.05. n = 3 for each group. (b) Alizarin red staining of CKD rat abdominal aortas and CKD patient radial arteries. (c) Representative immunohistochemical staining of USP47 in aortas of CKD rats and in the radial arteries of CKD patients. Arrows mark USP47 positive areas. The scale bar corresponds to sizes from 20 to 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35509185), licensed under a CC-BY license. Not internally tested by Novus Biologicals.