USP53 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00054532-B01P

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
View all USP53 Primary Antibodies »

Product Specifications

Immunogen

USP53 (AAH17382.1, 1 a.a. - 188 a.a.) full-length human protein. MDQCYFENSLSTECIIRSASRSDGCQMPKLFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLEVSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPNETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS

Specificity

USP53 - ubiquitin specific peptidase 53,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for USP53 Antibody - Azide and BSA Free

Western Blot: USP53 Antibody [H00054532-B01P]

Western Blot: USP53 Antibody [H00054532-B01P]

Western Blot: USP53 Antibody [H00054532-B01P] - Analysis of USP53 expression in transfected 293T cell line by USP53 polyclonal antibody. Lane 1: USP53 transfected lysate(20.68 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: USP53 Antibody [H00054532-B01P]

Immunocytochemistry/ Immunofluorescence: USP53 Antibody [H00054532-B01P]

Immunocytochemistry/Immunofluorescence: USP53 Antibody [H00054532-B01P] - Analysis of purified antibody to USP53 on HepG2 cell. (antibody concentration 10 ug/ml)
USP53 Antibody - Azide and BSA Free

Western Blot: USP53 Antibody - Azide and BSA Free [H00054532-B01P] -

Effect of EIF4A3 on USP53 expression. (A) The ENCORI websites analyzed the interaction of USP53 and EIF4A3. (B) The interaction between USP53 and EIF4A3 was detected by Co-IP. (C) BMSCs were transfected with sh-NC/sh-EIF4A3/pcDNA/EIF4A3 overexpression vector, and WB analysis was used to determine EIF4A3 protein level. (D, E) Act D assay was performed to measure the stability of USP53 mRNA. (F) WB analysis for the detection of USP53 protein expression. *P < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40481141), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
USP53 Antibody - Azide and BSA Free

Western Blot: USP53 Antibody - Azide and BSA Free [H00054532-B01P] -

Effect of EIF4A3 on USP53 expression. (A) The ENCORI websites analyzed the interaction of USP53 and EIF4A3. (B) The interaction between USP53 and EIF4A3 was detected by Co-IP. (C) BMSCs were transfected with sh-NC/sh-EIF4A3/pcDNA/EIF4A3 overexpression vector, and WB analysis was used to determine EIF4A3 protein level. (D, E) Act D assay was performed to measure the stability of USP53 mRNA. (F) WB analysis for the detection of USP53 protein expression. *P < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40481141), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for USP53 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. Has also been used for immunofluorescence.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: USP53

USP53 has no peptidase activity

Long Name

Inactive ubiquitin carboxyl-terminal hydrolase 53

Alternate Names

KIAA1350

Entrez Gene IDs

54532 (Human)

Gene Symbol

USP53

UniProt

AAH17382.1 (Human)

Additional USP53 Products

Product Documents for USP53 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for USP53 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for USP53 Antibody - Azide and BSA Free

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Protocols

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