USP7 Antibody (6512) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43619

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chicken (93%), Mouse (97%), Porcine (97%), Rat (98%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 6512

Format

Azide and BSA Free
View all USP7 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human USP7. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (93%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

128 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP7 Antibody (6512) - Azide and BSA Free

Western Blot: USP7 Antibody (6512) [NBP2-43619]

Western Blot: USP7 Antibody (6512) [NBP2-43619]

Western Blot: USP7 Antibody (6512) [NBP2-43619] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract 5 % SDS-PAGE USP7 antibody [6512] dilution: 1:1000
USP7 Antibody (6512) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: USP7 Antibody (6512) [NBP2-43619] -

USP7 antibody [GT6512] detects USP7 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP7 protein stained by USP7 antibody [GT6512] (NBP2-43619) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for USP7 Antibody (6512) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: USP7

HAUSP (also known as deubiquitinating enzyme 7, herpes virus associated ubiquitin specific protease, TEF1, ubiquitin carboxyl terminal hydrolase 7, ubiquitin specific protease 7, ubiquitin thiolesterase 7, and USP7) is a novel p53 interacting protein. HAUSP was identified by mass spectrometry of affinity-purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53 even in the presence of excess MDM2, and also induces p53-dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically de-ubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.

Long Name

Ubiquitin Specific Protease 7

Alternate Names

HAUSP, TEF1

Gene Symbol

USP7

Additional USP7 Products

Product Documents for USP7 Antibody (6512) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for USP7 Antibody (6512) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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