USP7 Antibody (OTI1F12)

Novus Biologicals | Catalog # NBP2-01544

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F12
View all USP7 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human USP7(NP_003461) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

128.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP7 Antibody (OTI1F12)

Western Blot: USP7 Antibody (OTI1F12) [NBP2-01544]

Western Blot: USP7 Antibody (OTI1F12) [NBP2-01544]

Western Blot: USP7 Antibody (1F12) [NBP2-01544] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP7.
Immunohistochemistry-Paraffin: USP7 Antibody (OTI1F12) [NBP2-01544]

Immunohistochemistry-Paraffin: USP7 Antibody (OTI1F12) [NBP2-01544]

Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human prostate tissue using anti-USP7 mouse monoclonal antibody.
Flow Cytometry: USP7 Antibody (OTI1F12) [NBP2-01544]

Flow Cytometry: USP7 Antibody (OTI1F12) [NBP2-01544]

Flow Cytometry: USP7 Antibody (1F12) [NBP2-01544] - Analysis of Hela cells, using anti-USP7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: USP7 Antibody (OTI1F12) [NBP2-01544]

Immunohistochemistry-Paraffin: USP7 Antibody (OTI1F12) [NBP2-01544]

Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-USP7 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP7 Antibody (OTI1F12) [NBP2-01544]

Immunohistochemistry-Paraffin: USP7 Antibody (OTI1F12) [NBP2-01544]

Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human colon tissue using anti-USP7 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP7 Antibody (OTI1F12) [NBP2-01544]

Immunohistochemistry-Paraffin: USP7 Antibody (OTI1F12) [NBP2-01544]

Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human Kidney tissue using anti-USP7 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP7 Antibody (OTI1F12) [NBP2-01544]

Immunohistochemistry-Paraffin: USP7 Antibody (OTI1F12) [NBP2-01544]

Immunohistochemistry-Paraffin: USP7 Antibody (1F12) [NBP2-01544] - Staining of paraffin-embedded Human pancreas tissue using anti-USP7 mouse monoclonal antibody.

Applications for USP7 Antibody (OTI1F12)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.8 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP7

HAUSP (also known as deubiquitinating enzyme 7, herpes virus associated ubiquitin specific protease, TEF1, ubiquitin carboxyl terminal hydrolase 7, ubiquitin specific protease 7, ubiquitin thiolesterase 7, and USP7) is a novel p53 interacting protein. HAUSP was identified by mass spectrometry of affinity-purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53 even in the presence of excess MDM2, and also induces p53-dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically de-ubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.

Long Name

Ubiquitin Specific Protease 7

Alternate Names

HAUSP, TEF1

Entrez Gene IDs

7874 (Human)

Gene Symbol

USP7

Additional USP7 Products

Product Documents for USP7 Antibody (OTI1F12)

Certificate of Analysis

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Product Specific Notices for USP7 Antibody (OTI1F12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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