USP9x Antibody (OTI2G7)
Novus Biologicals | Catalog # NBP2-03824
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Monkey
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2G7
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 2246-2570 of human USP9X (NP_001034680) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for USP9x Antibody (OTI2G7)
Western Blot: USP9x Antibody (2G7) [NBP2-03824] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-USP9x monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry-Paraffin: USP9x Antibody (OTI2G7) [NBP2-03824]
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human prostate tissue using anti-USP9x mouse monoclonal antibody.Western Blot: USP9x Antibody (OTI2G7) [NBP2-03824]
Western Blot: USP9x Antibody (2G7) [NBP2-03824] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP9x (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP9x.Immunohistochemistry-Paraffin: USP9x Antibody (OTI2G7) [NBP2-03824]
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-USP9x mouse monoclonal antibody.Immunohistochemistry-Paraffin: USP9x Antibody (OTI2G7) [NBP2-03824]
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-USP9x mouse monoclonal antibody.Immunohistochemistry-Paraffin: USP9x Antibody (OTI2G7) [NBP2-03824]
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-USP9x mouse monoclonal antibody.Immunohistochemistry-Paraffin: USP9x Antibody (OTI2G7) [NBP2-03824]
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-USP9x mouse monoclonal antibody.Immunohistochemistry-Paraffin: USP9x Antibody (OTI2G7) [NBP2-03824]
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human Kidney tissue using anti-USP9x mouse monoclonal antibody.Immunohistochemistry-Paraffin: USP9x Antibody (OTI2G7) [NBP2-03824]
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human liver tissue using anti-USP9x mouse monoclonal antibody.Applications for USP9x Antibody (OTI2G7)
Application
Recommended Usage
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500-2000
Reviewed Applications
Read 1 review rated 4 using NBP2-03824 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: USP9x
Long Name
Ubiquitin-specific Protease 9, X-linked
Alternate Names
DFFRX, FAF, FAM
Entrez Gene IDs
8239 (Human)
Gene Symbol
USP9X
UniProt
Additional USP9x Products
Product Documents for USP9x Antibody (OTI2G7)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for USP9x Antibody (OTI2G7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for USP9x Antibody (OTI2G7)
Customer Reviews for USP9x Antibody (OTI2G7) (1)
4 out of 5
1 Customer Rating
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Application: Immunohistochemistry-ParaffinSample Tested: See PMID 24152793Species: HumanVerified Customer | Posted 12/29/2014
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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