V5 Epitope Tag Antibody [Biotin]

Name: V5 Epitope Tag Antibody [Biotin]
Brand: Novus Biologicals
SKU: NB600-380B
Price: 529 USD
Availability: InStock

Novus Biologicals | Catalog # NB600-380B

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Epitope Tag

Applications

ELISA, Immunoprecipitation

Label

Biotin

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Goats were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for V5 Epitope Tag Antibody [Biotin]

Western Blot: V5 Epitope Tag Antibody [Biotin] [NB600-380B] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000). Detection: Streptavidin-HRP (0.1 ug/ml) and chemiluminescence with an exposure time of 3 seconds.

Applications for V5 Epitope Tag Antibody [Biotin]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.2% BSA

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: V5 Epitope Tag

The V5 epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized V5 epitope represents amino acid residues 95 to 108, GKPIPNPLLGLDST, of RNA polymerase alpha subunit of simian virus 5.

Alternate Names

GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5

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Product Documents for V5 Epitope Tag Antibody [Biotin]

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Certificate of Analysis

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Product Specific Notices for V5 Epitope Tag Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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