V5 Epitope Tag Antibody - BSA Free

Novus Biologicals | Catalog # NB600-380

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Epitope Tag

Cited:

Human, Rat

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all V5 Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Goats were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Reactivity Notes

Human reactivity reported in multiple pieces of scientific literature.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for V5 Epitope Tag Antibody - BSA Free

Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-380]

Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-380]

Western Blot: V5 Epitope Tag Antibody [NB600-380] - Detection of V5-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-V5 antibody NB600-380 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for V5 Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

Primary-1:1000-1:30000; Coating plates-1:100-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Immunoprecipitation

1 - 4ug/mg lysate

Western Blot

1:1000 - 1:30000
Application Notes
Use in immunprecipitation reported in scientific literature (PMID 25767115).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: V5 Epitope Tag

The V5 epitope tag is derived from an epitope present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). Usually the V5 tag is used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Alternate Names

GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5

Additional V5 Epitope Tag Products

Product Documents for V5 Epitope Tag Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for V5 Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for V5 Epitope Tag Antibody - BSA Free

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Protocols

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