VAMP-5 Antibody (3E5) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13515
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 3E5
Format
Azide and BSA Free
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Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to human VAMP-5.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Centrifuge briefly prior to opening.
Scientific Data Images for VAMP-5 Antibody (3E5) - Azide and BSA Free
Western Blot: VAMP-5 Antibody (3E5) [NBP3-13515] -
Western Blot: VAMP-5 Antibody (3E5) [NBP3-13515] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-5 antibody [3E5] (NBP3-13515) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: VAMP-5 Antibody (3E5) [NBP3-13515] -
ICC/IF analysis of pLEGFP-C1-hV5p transfected HeLa cells using NBP3-13515 VAMP5 antibody [3E5].Red : Primary antibody
Immunocytochemistry/ Immunofluorescence: VAMP-5 Antibody (3E5) [NBP3-13515] -
ICC/IF analysis of transfected NRK cells (V5V4-GFP) using NBP3-13515 VAMP5 antibody [3E5].Red : Primary antibody
Applications for VAMP-5 Antibody (3E5) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VAMP-5
Long Name
Vesicle-Associated Membrane Protein 5
Alternate Names
Myobrevin, VAMP5
Gene Symbol
VAMP5
Additional VAMP-5 Products
Product Documents for VAMP-5 Antibody (3E5) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VAMP-5 Antibody (3E5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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