VE-Cadherin Antibody (55-7H1) - BSA Free

Name: VE-Cadherin Antibody (55-7H1) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-09157
Price: 369 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-09157

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Product Documents

Clone 55-7H1 was used by HLDA to establish CD designation.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 55-7H1

Format

BSA Free

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Product Specifications

Immunogen

Human endothelial cells

Specificity

The mouse monoclonal antibody 55-7H1 recognizes a calcium-independent extracellular epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for VE-Cadherin Antibody (55-7H1) - BSA Free

Flow Cytometry: VE-Cadherin Antibody (55-7H1) - BSA Free [NBP3-09157]

Separation of HUVEC cells stained using anti-CD144 (55-7H1) purified antibody (concentration in sample 5 ug/ml, GAM APC, red-filled) from HUVEC cells unstained by primary antibody (GAM APC, black-dashed) in Analysis (surface staining).

Applications for VE-Cadherin Antibody (55-7H1) - BSA Free

Application

Recommended Usage

Flow Cytometry

1-4 ug/ml

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VE-Cadherin

VE-cadherin (cadherin-5) is an endothelial specific adhesion molecule that is essential for the maintenance of endothelial barrier function and angiogenesis. VE-cadherin is linked to the actin cytoskeleton through a number of adaptor proteins including a-, â-, and ã-catenin. Cytoskeletal dynamics and phosphorylation regulate VE-cadherin mediated cell-cell adhesion. The cytosolic C-terminal Src kinase (Csk) binds via its SH2 domain to the phosphorylated tyrosine 658 site of VE-cadherin. This interaction regulates density dependent cell proliferation. Phosphorylation of tyrosine 658 has also been shown to lead to the uncoupling of p120-catenin from the cytoplasmic tail of VE-cadherin. This phosphorylation event is sufficient to maintain cells in a mesenchymal state during the cell invasion phase of angiogenesis.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Gene Symbol

CDH5

Additional VE-Cadherin Products

Product Documents for VE-Cadherin Antibody (55-7H1) - BSA Free

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Certificate of Analysis

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Product Specific Notices for VE-Cadherin Antibody (55-7H1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.