VectaCell Trolox Antifade Reagent for Live Cell Imaging
Novus Biologicals | Catalog # CB-1000-2-NB
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Key Product Details
Applications
Immunocytochemistry/ Immunofluorescence, Microscopy
Product Summary for VectaCell Trolox Antifade Reagent for Live Cell Imaging
VectaCell Trolox is an antifading additive for live cell imaging. It is a stock solution (100 mM) of the antioxidant Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) in filtered ethanol. Trolox is a water-soluble and cell-permeable analog of vitamin E that efficiently prevents formation of various reactive oxygen species, such as singlet oxygen (O2), superoxide anion (O2-) or hydrogen peroxide (H2O2).
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Product Specifications
Application Notes
Dilute the Trolox Solution into culture media or imaging buffer to a final concentration between 0.1 mM and 1 mM. The optimal working concentration of Trolox in imaging media will depend on the cell type and their tolerance to hypoxia. For cells that have low tolerance to hypoxia, or can be affected by the addition of ethanol, the appropriate controls should be included in the assay..
Scientific Data Images for VectaCell Trolox Antifade Reagent for Live Cell Imaging
[CB-1000-2-NB]
Formulation, Preparation, and Storage
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4°C.
Product Documents for VectaCell Trolox Antifade Reagent for Live Cell Imaging
Certificate of Analysis
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Product Specific Notices for VectaCell Trolox Antifade Reagent for Live Cell Imaging
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Citations for VectaCell Trolox Antifade Reagent for Live Cell Imaging
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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