VGLUT1 Antibody (S28-9) [Alexa Fluor® 405]

Novus Biologicals | Catalog # NBP2-59329AF405

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunocytochemistry/ Immunofluorescence

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # S28-9
View all VGLUT1/SLC17A7 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

Localization

Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse

Specificity

Detects 52kDa. No cross-reactivity against VGlut2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for VGLUT1 Antibody (S28-9) [Alexa Fluor® 405]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-59329AF405 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: VGLUT1/SLC17A7

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Long Name

Vesicular Glutamate Transporter 1

Alternate Names

BNPI, SLC17A7

Gene Symbol

SLC17A7

Additional VGLUT1/SLC17A7 Products

Product Documents for VGLUT1 Antibody (S28-9) [Alexa Fluor® 405]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for VGLUT1 Antibody (S28-9) [Alexa Fluor® 405]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

Customer Reviews for VGLUT1 Antibody (S28-9) [Alexa Fluor® 405] (1)

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  • VGLUT1 Antibody (S28-9) [Alexa Fluor® 405]
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: Adult mouse brain tissue and Mouse brain
    Species: Mouse
    Verified Customer | Posted 07/19/2021
    Acutely isolated CD45mid/CD11b+ microglia demonstrate VGLUT1+ positivity. Positive control staining is seen in the CD45-/CD11b- population which includes neuronal fragments and other glia.
    Single cell suspensions were prepared from mouse brain from 1 year old 5xFAD mice using enzymatic digestion and a 27% percoll gradient to clear myelin. Cells were then stained for CD45 and CD11b, fixed and permeabilized, and then stained with the ant-vglut1 antibody for 30 minutes at room temperature.
    VGLUT1 Antibody (S28-9) [Alexa Fluor® 405] NBP2-59329AF405
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs

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