VGLUT1 Antibody

Novus Biologicals | Catalog # NBP1-20267

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Immunogen

A synthetic peptide from amino acid region 500-560 of rat VGLUT1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and human.

Specificity

VGLUT1 (both isoforms 1 and 2).

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for VGLUT1 Antibody

Application
Recommended Usage

Flow Cytometry

1: 300 - 1: 2000

Immunohistochemistry

1:300-1:2000

Western Blot

1:300-1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VGLUT1/SLC17A7

FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.; SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.

Long Name

Vesicular Glutamate Transporter 1

Alternate Names

BNPI, SLC17A7

Entrez Gene IDs

57030 (Human); 72961 (Mouse); 116638 (Rat)

Gene Symbol

SLC17A7

UniProt

Additional VGLUT1/SLC17A7 Products

Product Documents for VGLUT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for VGLUT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VGLUT1 Antibody

Showing  1 - 2 of 2 FAQs Showing All
  • Q: I would like to know if your VGLUT1 antibody (NBP1-20267) has ever been validated for IHC and IF using mouse samples? If so, would you please provide the IHC image?

    A:

    We do not have any images available for NBP1-20267 and it has not been validated or tested for use in Immunofluorescence. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.

  • Q: What is the isotype of vGlut1 primary antibody. NBP1-20267?

    A:

    This antibody is a sheep polyclonal antibody and is supplied as whole antisera. Therefore is a mixture of all isotypes as well as other proteins. You can perform a purification step if needed. Please see 860-0005 as a kit that will purify out IgG isotype

  • Q: I would like to know if your VGLUT1 antibody (NBP1-20267) has ever been validated for IHC and IF using mouse samples? If so, would you please provide the IHC image?

    A:

    We do not have any images available for NBP1-20267 and it has not been validated or tested for use in Immunofluorescence. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.

  • Q: What is the isotype of vGlut1 primary antibody. NBP1-20267?

    A:

    This antibody is a sheep polyclonal antibody and is supplied as whole antisera. Therefore is a mixture of all isotypes as well as other proteins. You can perform a purification step if needed. Please see 860-0005 as a kit that will purify out IgG isotype

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