VGLUT2 Antibody (CL2952) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43789
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat, Rhesus Macaque
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL2952
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of Q9P2U8, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Use in Rhesus Macaque reported in scientific publication (PMID: 32337749) used in alternate format NBP2-46641.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for VGLUT2 Antibody (CL2952) - Azide and BSA Free
![VGLUT2 Antibody (CL2952) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]](https://resources.rndsystems.com/images/products/nbp3-43789_mouse-vglut2-mab-cl2952-azide-and-bsa-free-28220251210423.jpg "Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]")
![VGLUT2 Antibody (CL2952) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]](https://resources.rndsystems.com/images/products/nbp3-43789_mouse-vglut2-mab-cl2952-azide-and-bsa-free-282202512394455.jpg "Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]")
Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]
Staining of human cerebellum shows strong positivity in glutamatergic fibers in molecular and granular layers.![VGLUT2 Antibody (CL2952) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]](https://resources.rndsystems.com/images/products/nbp3-43789_mouse-vglut2-mab-cl2952-azide-and-bsa-free-282202512405598.jpg "Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]")
Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]
Staining of human cerebral cortex shows strong positivity in neuropil.![VGLUT2 Antibody (CL2952) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]](https://resources.rndsystems.com/images/products/nbp3-43789_mouse-vglut2-mab-cl2952-azide-and-bsa-free-282202513401747.jpg "Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]")
Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]
Staining of rat brain shows strong positivity in glutamatergic synapses in the thalamus.![VGLUT2 Antibody (CL2952) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]](https://resources.rndsystems.com/images/products/nbp3-43789_mouse-vglut2-mab-cl2952-azide-and-bsa-free-282202513254752.jpg "Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]")
Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]
Staining of mouse cerebellum shows moderate to strong positivity in glutamatergic fibers in granular and molecular layers..![VGLUT2 Antibody (CL2952) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]](https://resources.rndsystems.com/images/products/nbp3-43789_mouse-vglut2-mab-cl2952-azide-and-bsa-free-282202513401735.jpg "Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]")
Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]
Staining of rat thalamus shows strong immunoreactivity in glutamatergic synapses.![VGLUT2 Antibody (CL2952) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]](https://resources.rndsystems.com/images/products/nbp3-43789_mouse-vglut2-mab-cl2952-azide-and-bsa-free-28220251337818.jpg "Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]")
Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]
Staining of rat brain shows moderate to strong positivity in glutamatergic fibers in granular and molecular layers of the cerebellum.![VGLUT2 Antibody (CL2952) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]](https://resources.rndsystems.com/images/products/nbp3-43789_mouse-vglut2-mab-cl2952-azide-and-bsa-free-282202513261998.jpg "Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]")
Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]
Staining of mouse olfactory bulb shows strong positivity in glutamatergic fibers in the glomerular layer.![VGLUT2 Antibody (CL2952) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]](https://resources.rndsystems.com/images/products/nbp3-43789_mouse-vglut2-mab-cl2952-azide-and-bsa-free-28220251324557.jpg "Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]")
Immunohistochemistry-Paraffin: VGLUT2 Antibody [NBP3-43789]
Staining of human tonsil shows no positivity in lymphoid cells as expected.Applications for VGLUT2 Antibody (CL2952) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: VGLUT2
Alternate Names
differentiation-associated BNPI, differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, differentiation-associated Na-dependent inorganic phosphate cotransporter, DNPI, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, solute carrier family 17 member 6, vesicular glutamate transporter 2, VGLUT2
Gene Symbol
SLC17A6
Additional VGLUT2 Products
Product Documents for VGLUT2 Antibody (CL2952) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VGLUT2 Antibody (CL2952) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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