Vinculin Antibody (23GB2205)

Novus Biologicals | Catalog # NBP3-51026

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2205
View all Vinculin Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Vinculin (Uniprot: P18206)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Vinculin Antibody (23GB2205)

Vinculin Antibody (23GB2205)

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (23GB2205)[NBP3-51026] -

Immunocytochemical staining of HT-1080 cells with Vinculin antibody (1:1,000). Nuclei were stained blue with DAPI; Vinculin was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Vinculin Antibody (23GB2205)

Flow Cytometry: Vinculin Antibody (23GB2205)[NBP3-51026] -

Flow cytometric analysis of Vinculinexpression in HT-1080 cells using Vinculin antibody (1:2,000). Green, isotype control; red, Vinculin.
Vinculin Antibody (23GB2205)

Western Blot: Vinculin Antibody (23GB2205)[NBP3-51026] -

Western blotting analysis using Vinculin antibody (1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
Vinculin Antibody (23GB2205)

Western Blot: Vinculin Antibody (23GB2205)[NBP3-51026] -

Western blotting analysis using Vinculin antibody (1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for Vinculin Antibody (23GB2205)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Vinculin

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. (provided by RefSeq)

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Gene Symbol

VCL

Additional Vinculin Products

Product Documents for Vinculin Antibody (23GB2205)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Vinculin Antibody (23GB2205)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Vinculin Antibody (23GB2205)

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  • Q: Do you have any 100 coda or bigger controls?

    A:

    I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

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