Vinculin Antibody (VIN-11-5)

Novus Biologicals | Catalog # NB120-11193

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Key Product Details

Species Reactivity

Human, Mouse, Bovine, Chicken

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Microarray

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # VIN-11-5
View all Vinculin Primary Antibodies »

Product Specifications

Immunogen

Smooth muscle vinculin from chicken gizzard.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Marker

Focal Adhesion Marker

Specificity

The reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Vinculin Antibody (VIN-11-5)

Western Blot: Vinculin Antibody (VIN-11-5) [NB120-11193]

Western Blot: Vinculin Antibody (VIN-11-5) [NB120-11193]

Western Blot: Vinculin Antibody (VIN-11-5) [NB120-11193] - Chicken gizzard cell extract was separated on SDS-PAGE and probed with NB120-11193. The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBT/BCIP substrate. Antibody dilution 1/200.
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (VIN-11-5) [NB120-11193]

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (VIN-11-5) [NB120-11193]

Immunocytochemistry/Immunofluorescence: Vinculin Antibody (VIN-11-5) [NB120-11193] - Double labeling of an isolated smooth muscle cell (chicken gizzard) with Monoclonal Anti-Vinculin, clone no. VIN-11-5 (green) and Anti-alpha Actinin (red).

Applications for Vinculin Antibody (VIN-11-5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vinculin

Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell extracellular matrix adherens type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. The sequences of chicken, nematode and human vinculin have been determined. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha actinin and possibly with itself. Since alpha actinin binds to and cross links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Entrez Gene IDs

7414 (Human); 22330 (Mouse); 396422 (Chicken)

Gene Symbol

VCL

UniProt

P12003 (Chicken)

Additional Vinculin Products

Product Documents for Vinculin Antibody (VIN-11-5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Vinculin Antibody (VIN-11-5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Vinculin Antibody (VIN-11-5)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Vinculin Antibody (VIN-11-5)

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  • Q: Do you have any 100 coda or bigger controls?

    A:

    I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

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