VPS34 Antibody (9U5O3)

Novus Biologicals | Catalog # NBP3-15441

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9U5O3 expressed in HEK293
View all VPS34/PIK3C3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 788-887 of human VPS34 (Q8NEB9). GGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS34 Antibody (9U5O3)

Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441]

Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441]

Western Blot: VPS34 Antibody (9U5O3) [NBP3-15441] - Western blot analysis of extracts of various cell lines, using VPS34 Rabbit mAb (NBP3-15441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [NBP3-15441]

Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [NBP3-15441]

Immunocytochemistry/Immunofluorescence: VPS34 Antibody (9U5O3) [NBP3-15441] - Immunofluorescence analysis of NIH-3T3 cells using VPS34 Rabbit mAb (NBP3-15441) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation: VPS34 Antibody (9U5O3) [NBP3-15441]

Immunoprecipitation: VPS34 Antibody (9U5O3) [NBP3-15441]

Immunoprecipitation: VPS34 Antibody (9U5O3) [NBP3-15441] - Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug VPS34 antibody (NBP3-15441). Western blot was performed from the immunoprecipitate using VPS34 antibody (NBP3-15441) at a dilition of 1:500.
VPS34 Antibody (9U5O3)

Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [VPS34] -

Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [VPS34] - Confocal imaging of C2C12 cells using VPS34 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
VPS34 Antibody (9U5O3)

Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [VPS34] -

Immunocytochemistry/ Immunofluorescence: VPS34 Antibody (9U5O3) [VPS34] - Confocal imaging of PC-3 cells using VPS34 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for VPS34 Antibody (9U5O3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS34/PIK3C3

VPS34, also known as phosphoinositide-3-kinase p100 subunit or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. VPS34 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. It exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. The activity of VPS34 is enhanced by manganese and can be regulated by nutrients, suggesting an important role of VPS34 in the regulation of mTOR protein synthesis. VPS34 also forms a complex with Beclin1 that promotes autophagy and tumor supression.

Alternate Names

PI 3-Kinase C3, PI3K type 3, PIK3C3

Gene Symbol

PIK3C3

Additional VPS34/PIK3C3 Products

Product Documents for VPS34 Antibody (9U5O3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VPS34 Antibody (9U5O3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for VPS34 Antibody (9U5O3)

There are currently no reviews for this product. Be the first to review VPS34 Antibody (9U5O3) and earn rewards!

Have you used VPS34 Antibody (9U5O3)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies