VPS35 Antibody (11H10) - BSA Free
Novus Biologicals | Catalog # NBP2-78827
![Western Blot: VPS35 Antibody (11H10) [NBP2-78827]](https://resources.rndsystems.com/images/products/VPS35-Antibody-11H10-Western-Blot-NBP2-78827-img0012.jpg "Western Blot: VPS35 Antibody (11H10) [NBP2-78827]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 11H10
Format
BSA Free
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Product Specifications
Immunogen
Full length recombinant human VSP35
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for VPS35 Antibody (11H10) - BSA Free
Western Blot: VPS35 Antibody (11H10) [NBP2-78827]
Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.![Immunohistochemistry: VPS35 Antibody (11H10) [NBP2-78827]](https://resources.rndsystems.com/images/products/VPS35-Antibody-11H10-Immunohistochemistry-NBP2-78827-img0013.jpg "Immunohistochemistry: VPS35 Antibody (11H10) [NBP2-78827]")
Immunohistochemistry: VPS35 Antibody (11H10) [NBP2-78827]
Immunohistochemistry: VPS35 Antibody (11H10) [NBP2-78827] - Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Tissue: Intestinal Cancer. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.![Western Blot: VPS35 Antibody (11H10) [NBP2-78827]](https://resources.rndsystems.com/images/products/VPS35-Antibody-11H10-Western-Blot-NBP2-78827-img0011.jpg "Western Blot: VPS35 Antibody (11H10) [NBP2-78827]")
Western Blot: VPS35 Antibody (11H10) [NBP2-78827]
Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]](https://resources.rndsystems.com/images/products/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0007.jpg "Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]")
Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]
Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. clone 11H10 depletes VPS35 from the A549 cell extract.![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]](https://resources.rndsystems.com/images/products/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0008.jpg "Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]")
Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]
Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]](https://resources.rndsystems.com/images/products/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0009.jpg "Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]")
Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]
Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]](https://resources.rndsystems.com/images/products/VPS35-Antibody-11H10-Immunoprecipitation-NBP2-78827-img0010.jpg "Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]")
Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827]
Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of MEF lysate for 2 hours at 4C.Applications for VPS35 Antibody (11H10) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100
Immunoprecipitation
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 °C.
Background: VPS35
Long Name
VPS35 retromer complex component
Alternate Names
MEM3, PARK17
Gene Symbol
VPS35
Additional VPS35 Products
Product Documents for VPS35 Antibody (11H10) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VPS35 Antibody (11H10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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