VPS35 Antibody (7E4) - BSA Free

Novus Biologicals | Catalog # NBP2-78823

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 7E4

Format

BSA Free
View all VPS35 Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human VSP35

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for VPS35 Antibody (7E4) - BSA Free

Western Blot: VPS35 Antibody (7E4) [NBP2-78823]

Western Blot: VPS35 Antibody (7E4) [NBP2-78823]

Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.
Western Blot: VPS35 Antibody (7E4) [NBP2-78823]

Western Blot: VPS35 Antibody (7E4) [NBP2-78823]

Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.
Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823]

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823]

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823). Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.
Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823]

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823]

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. Clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract.
Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823]

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823]

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.
Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823]

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823]

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.
Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823]

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823]

Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: MEF, A549 cells. Species: Human, Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (SMC-602) at 1:5 (tissue culture supernatant). 10 ug antibody were coupled to 10 uL A/G resin beads either covalently (with DMP) or non-covalently (1 hour at 4 degrees). The antibody immunoprecipitates VPS35 in mouse and human cells effectively when covalently coupled to the beads..

Applications for VPS35 Antibody (7E4) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunoprecipitation

1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: VPS35

The maintenance of organelles in eukaryotic cells depends on sorting proteins, which ensure the proper delivery of organelle-specific proteins. In S. cerevisiae, VPS35 has a direct role in the retrieval of vacuolar cargo proteins, suggesting that human VPS35 may have an analogous function in the maintenance of lysosomes.

Long Name

VPS35 retromer complex component

Alternate Names

MEM3, PARK17

Gene Symbol

VPS35

Additional VPS35 Products

Product Documents for VPS35 Antibody (7E4) - BSA Free

Certificate of Analysis

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Product Specific Notices for VPS35 Antibody (7E4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VPS35 Antibody (7E4) - BSA Free

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Protocols

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