Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WDR45 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide from the C-terminal of Human WDR45(aa. 312-322)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR45 Antibody - BSA Free

Western Blot: WDR45 Antibody [NBP3-18255]

Western Blot: WDR45 Antibody [NBP3-18255]

Western Blot: WDR45 Antibody [NBP3-18255] - Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 40 kDa WDR45 protein using Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 40 kDa.
Immunocytochemistry/ Immunofluorescence: WDR45 Antibody [NBP3-18255]

Immunocytochemistry/ Immunofluorescence: WDR45 Antibody [NBP3-18255]

Immunocytochemistry/Immunofluorescence: WDR45 Antibody [NBP3-18255] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody (NBP3-18255) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WDR45 Antibody (D) Composite.

Applications for WDR45 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS, 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WDR45

WDR45 encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined. [provided by RefSeq]

Alternate Names

JM5, WD repeat domain 45, WD repeat domain phosphoinositide-interacting protein 4, WD repeat-containing protein 45, WD45 repeat protein interacting with phosphoinositides 4, WDRX1, WDRXI4, WIPI-4, X-linked 1

Gene Symbol

WDR45

Additional WDR45 Products

Product Documents for WDR45 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WDR45 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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